Journal: Oncology Letters

Article Title: Solitary fibrous tumor of the brain: A report of 3 cases

doi: 10.3892/ol.2024.14621

Figure Lengend Snippet: Clinicopathological parameters of the reported cases.

Article Snippet: Immunostaining for STAT6 (rabbit monoclonal antibody clone EP325; 1:100; cat. no. BSB-3809-05; Bio SB Inc.), CD34 (rabbit monoclonal antibody clone EP88; 1:100; cat. no. 134R-16-RUO; Cell Marque; Merck KGaA) and Ki67 (mouse monoclonal antibody clone MIB-1; 1:100; cat. no. Z2305ML; Zeta Corporation) at 37°C for 44 min was conducted using a Ventana BenchMark XT 750-700 Automated IHC/ISH autostainer (Roche Diagnostics, Ltd.) was performed as per the manufacturer's protocol, using heat-induced antigen retrieval (100°C, 4 min) and endogenous peroxidase blocking by 0.04% hydrogen peroxide.

Techniques: Imaging, Immunohistochemistry, Biomarker Discovery

Journal: Oncology Letters

Article Title: Solitary fibrous tumor of the brain: A report of 3 cases

doi: 10.3892/ol.2024.14621

Figure Lengend Snippet: Microscopic images of Case 1. (A) H&E-stained image (magnification, ×10). Tumor cells were observed in sheets with interspersed dilated vessels (*). (B) H&E-stained image (magnification, ×20). The arrow highlights the amianthoid fibers. (C) H&E-stained images (magnification, ×40). The arrow highlights the mitotic figures. (D) H&E-stained image (magnification, 10×), *indicates necrosis. Immunohistochemistry (DAB stain; magnification, ×20) for (E) CD34 and (F) STAT6 was positive. H&E, hematoxylin and eosin.

Article Snippet: Immunostaining for STAT6 (rabbit monoclonal antibody clone EP325; 1:100; cat. no. BSB-3809-05; Bio SB Inc.), CD34 (rabbit monoclonal antibody clone EP88; 1:100; cat. no. 134R-16-RUO; Cell Marque; Merck KGaA) and Ki67 (mouse monoclonal antibody clone MIB-1; 1:100; cat. no. Z2305ML; Zeta Corporation) at 37°C for 44 min was conducted using a Ventana BenchMark XT 750-700 Automated IHC/ISH autostainer (Roche Diagnostics, Ltd.) was performed as per the manufacturer's protocol, using heat-induced antigen retrieval (100°C, 4 min) and endogenous peroxidase blocking by 0.04% hydrogen peroxide.

Techniques: Staining, Immunohistochemistry

Journal: Oncology Letters

Article Title: Solitary fibrous tumor of the brain: A report of 3 cases

doi: 10.3892/ol.2024.14621

Figure Lengend Snippet: Microscopic images of Case 2. (A) H&E-stained image (magnification, ×10). ‘Patternless’ architecture of tumor cells with a hemangiopericytomatous vasculature (*). (B) H&E-stained image (magnification, ×40). The arrow highlights mitotic figures. Immunohistochemistry (DAB stain; magnification, ×10) for (C) CD34 was negative, while (D) STAT6 was positive in the tumor cells. H&E, hematoxylin and eosin.

Article Snippet: Immunostaining for STAT6 (rabbit monoclonal antibody clone EP325; 1:100; cat. no. BSB-3809-05; Bio SB Inc.), CD34 (rabbit monoclonal antibody clone EP88; 1:100; cat. no. 134R-16-RUO; Cell Marque; Merck KGaA) and Ki67 (mouse monoclonal antibody clone MIB-1; 1:100; cat. no. Z2305ML; Zeta Corporation) at 37°C for 44 min was conducted using a Ventana BenchMark XT 750-700 Automated IHC/ISH autostainer (Roche Diagnostics, Ltd.) was performed as per the manufacturer's protocol, using heat-induced antigen retrieval (100°C, 4 min) and endogenous peroxidase blocking by 0.04% hydrogen peroxide.

Techniques: Staining, Immunohistochemistry

Journal: Oncology Letters

Article Title: Solitary fibrous tumor of the brain: A report of 3 cases

doi: 10.3892/ol.2024.14621

Figure Lengend Snippet: Microscopic images of subpleural metastatic nodule in Case 3. (A) H&E-stained image (magnification, ×10). Low-power magnification of fibromuscular tissue infiltrated by tumor cells. (B) H&E-stained image (magnification, ×20). *marks the area with stromal hyalinization. Immunohistochemistry (DAB stain; magnification, ×20) for (C) CD34 shows heterogenous positivity, while (D) STAT6 shows diffuse positive expression. H&E, hematoxylin and eosin.

Article Snippet: Immunostaining for STAT6 (rabbit monoclonal antibody clone EP325; 1:100; cat. no. BSB-3809-05; Bio SB Inc.), CD34 (rabbit monoclonal antibody clone EP88; 1:100; cat. no. 134R-16-RUO; Cell Marque; Merck KGaA) and Ki67 (mouse monoclonal antibody clone MIB-1; 1:100; cat. no. Z2305ML; Zeta Corporation) at 37°C for 44 min was conducted using a Ventana BenchMark XT 750-700 Automated IHC/ISH autostainer (Roche Diagnostics, Ltd.) was performed as per the manufacturer's protocol, using heat-induced antigen retrieval (100°C, 4 min) and endogenous peroxidase blocking by 0.04% hydrogen peroxide.

Techniques: Staining, Immunohistochemistry, Expressing

Journal: Aging (Albany NY)

Article Title: Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation

doi: 10.18632/aging.102613

Figure Lengend Snippet: Rapamycin could protect mice from bleomycin (BLM)-induced pulmonary fibrosis. Mice (n = 10 in each group) were intraperitoneally injected with vehicle (DMSO/PBS, 10%) or 5 mg/kg rapamycin every other day starting 7 days after administration of BLM (5 mg/kg). ( A ) Pulmonary fibrosis was determined by haematoxylin and eosin (H&E) staining. Collagen was revealed by Masson’s trichrome staining. The expression of p21 was measured by immunohistochemical analysis. ( B ) The protein levels of p16, p21, α-SMA and collagen I were detected by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, *P < 0.05 and **P < 0.01. ( C , D ) The lung tissues were double stained with E-cadherin and p21 (C), α-SMA and collagen I (D) by immunofluorescence. The positive areas of p21 and collagen I were quantified by densitometry (n = 3), **P < 0.01.

Article Snippet: Rabbit monoclonal antibody against GAPDH was purchased from BOSTER (Wuhan, China).

Techniques: Injection, Staining, Expressing, Immunohistochemical staining, Western Blot, Control, Immunofluorescence

Journal: Aging (Albany NY)

Article Title: Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation

doi: 10.18632/aging.102613

Figure Lengend Snippet: Epithelial cell senescence could induce pulmonary fibroblast activation via activating Wnt/β-catenin signalling. ( A – C ) MLE-12 cells were treated with bleomycin (BLM, 25 μg/ml) for the indicated times. (A, B) SA-β-gal staining and β-galactosidase activity measurement were performed to detect cellular senescence, * P < 0.05 and ** P < 0.01 vs. 0 h. ( C ) The protein levels of p21 and p16 were measured by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, *P < 0.05 and **P < 0.01. ( D – I ) MLE-12 cells were pre-treated with or without BLM for 3 days. The medium was replaced by fresh medium without BLM and co-cultured with pulmonary fibroblasts for another 3 days. ( D ) The migration capacity of pulmonary fibroblasts was detected by using a wound-healing assay. Wound areas were calculated by ImageJ, **P < 0.01. ( E ) The proliferation ability of pulmonary fibroblasts was measured by EdU assay. The percentage of proliferating cells were calculated by ImageJ, **P < 0.01. ( F ) The protein levels of collagen I, vimentin and α-SMA were determined by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, **P < 0.01. ( G ) Pulmonary fibroblasts were double stained with vimentin and collagen I by immunofluorescence. ( H ) Pulmonary fibroblasts were double stained with α-SMA and periostin by immunofluorescence. ( I ) The expression of β-catenin was measured by immunofluorescence. ( J ) MLE-12 cells were pre-treated with or without BLM for 3 days. MLE-12 cells were cultured with fresh medium without BLM for another 3 days. The supernatants were collected to culture pulmonary fibroblasts in the presence or absence of ICG-001. The expression of β-catenin, α-SMA and collagen I were examined by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, *P < 0.05 and **P < 0.01.

Article Snippet: Rabbit monoclonal antibody against GAPDH was purchased from BOSTER (Wuhan, China).

Techniques: Activation Assay, Staining, Activity Assay, Western Blot, Expressing, Control, Cell Culture, Migration, Wound Healing Assay, EdU Assay, Immunofluorescence

Journal: Aging (Albany NY)

Article Title: Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation

doi: 10.18632/aging.102613

Figure Lengend Snippet: Rapamycin could suppress epithelial cell senescence and fibroblast activation via impairing the production of SASP. ( A – E ) MLE-12 cells were treated with bleomycin (BLM), followed by treatment with or without rapamycin for 3 days. ( A ) The expression of p16 and p21 were measured by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, **P < 0.01. ( B , C ) The protein levels of p16 and p21 were detected by immunofluorescence. ( D ) The mRNA levels of IL-1β, IL-6, IL-8 and TNF-α were determined by Q-PCR, ** P < 0.01 vs. Con and ## P < 0.01 vs. BLM. ( E , F ) MLE-12 cells were treated as in and co-cultured with pulmonary fibroblasts in fresh medium for another 3 days. ( E ) The proliferation ability of pulmonary fibroblasts were measured by EdU assay. The percentage of proliferating cells was calculated by ImageJ, **P < 0.01. ( F ) The expression of α-SMA and collagen I were detected by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, **P < 0.01.

Article Snippet: Rabbit monoclonal antibody against GAPDH was purchased from BOSTER (Wuhan, China).

Techniques: Activation Assay, Expressing, Western Blot, Control, Immunofluorescence, Cell Culture, EdU Assay

Journal: Aging (Albany NY)

Article Title: Epithelial cell senescence induces pulmonary fibrosis through Nanog-mediated fibroblast activation

doi: 10.18632/aging.102613

Figure Lengend Snippet: Nanog silencing could suppress pulmonary fibroblast activation and impair the development of pulmonary fibrosis. ( A – C ) Pulmonary fibroblasts were transfected with LV-Nanog-siRNA and co-cultured with MLE-12 cells as in . ( A ) The mRNA levels of Nanog, Oct4 and Rex1 were measured by Q-PCR, ** P < 0.01 vs. Con and ## P < 0.01 vs. bleomycin (BLM). ( B ) The protein levels of collagen I and α-SMA were determined by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, *P < 0.05 and **P < 0.01. ( C ) The expression of collagen I and α-SMA were further examined by immunofluorescence staining. ( D – G ) Mice were intratracheally injected with 5 × 10 8 TU/ml LV-Nanog-siRNA or negative control (NC) 7 days after administration of BLM. Mice were sacrificed on day 21 after BLM instillation. ( D ) Pulmonary fibrosis was determined by haematoxylin and eosin (H&E) staining and collagen I was revealed by Sirius Red/Fast Green staining. ( E ) The mRNA levels of Nanog, α-SMA and collagen I were determined by Q-PCR, ** P < 0.01 vs. NC and ## P < 0.01 vs. NC + BLM. ( F ) The protein levels of Nanog, collagen I and α-SMA were measured by Western blot. The expression levels were quantified with ImageJ (n = 3). GAPDH was used as a loading control, *P < 0.05 and **P < 0.01. ( G ) The expression of α-SMA and collagen I were further confirmed by immunofluorescence staining.

Article Snippet: Rabbit monoclonal antibody against GAPDH was purchased from BOSTER (Wuhan, China).

Techniques: Activation Assay, Transfection, Cell Culture, Western Blot, Expressing, Control, Immunofluorescence, Staining, Injection, Negative Control

Journal: Scientific reports

Article Title: Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1.

doi: 10.1038/s41598-023-34209-5

Figure Lengend Snippet: Figure 1. Expression of the GRK2 transgene. (A) Expression of the Grk2 transgene was determined by real- time PCR in the control (n = 12) and GRK2 groups (n = 11). (B) Representative immunohistochemical staining images of in vivo protein GRK2 levels in aortas from mice in both groups. Results are expressed as mean ± SEM. GRK2 G protein-coupled receptor kinase 2, rRNA ribosomal RNA, ns not significant. *P < 0.05, ***P < 0.001.

Article Snippet: GRK2 was detected in these sections using an anti-GRK2 rabbit monoclonal antibody (1:50, ST05-60; Novus, Littleton, Colorado, United States).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Immunohistochemical staining, Staining, In Vivo

Journal: Scientific reports

Article Title: Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1.

doi: 10.1038/s41598-023-34209-5

Figure Lengend Snippet: Figure 2. Baseline blood pressure and heart rate. (A) and (B) Baseline resting SBP, MAP, DBP and HR in the control (n = 13) and GRK2 (n = 12) groups. Results are expressed as mean ± SEM. Comparisons of parameters were performed using the 2-tailed Student t test or Welch test. DBP diastolic blood pressure, GRK2 G protein- coupled receptor kinase 2, HR heart rate, MAP mean arterial pressure, SBP systolic blood pressure, ns not significant. *P < 0.05.

Article Snippet: GRK2 was detected in these sections using an anti-GRK2 rabbit monoclonal antibody (1:50, ST05-60; Novus, Littleton, Colorado, United States).

Techniques: Control

Journal: Scientific reports

Article Title: Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1.

doi: 10.1038/s41598-023-34209-5

Figure Lengend Snippet: Figure 3. Blood pressure and heart rate pre and post either epinephrine or saline injection. SBP (A), MAP (B), DBP (C) and HR (D) in the control (n = 13) and GRK2 (n = 12) groups. (E) Ratio of pulmonary edema to body weight (Fluid in the lungs/BW) in the control (n = 12) and GRK2 (n = 11) groups. Results are expressed as mean ± SEM. Comparisons of parameters were performed using either the 2-tailed Student t test or Welch test, and repeated-measures 2-way ANOVA was used for multiple comparisons. BW body weight, DBP diastolic blood pressure, GRK2 G protein-coupled receptor kinase 2, HR heart rate, MAP mean arterial pressure, rRNA ribosomal RNA, SBP systolic blood pressure, ns not significant. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: GRK2 was detected in these sections using an anti-GRK2 rabbit monoclonal antibody (1:50, ST05-60; Novus, Littleton, Colorado, United States).

Techniques: Saline, Injection, Control

Journal: Scientific reports

Article Title: Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1.

doi: 10.1038/s41598-023-34209-5

Figure Lengend Snippet: Figure 4. Elevation of Nppb and LVEF was preserved in GRK2 overexpressing mice after epinephrine injection. (A) Left ventricular Nppb mRNA expression levels in the control (n = 10) and GRK2 (n = 7) groups. (B) Ratio of heart weight to body weight (HW/BW) in the control (n = 10) and GRK2 (n = 7) groups. (C) Percentage of LVEF in the control (n = 10) and GRK2 (n = 7) groups. (D) Representative Masson’s trichrome stain images in small coronary arteries in the myocardium of the left ventricle from mice in both groups. Results are expressed as mean ± SEM. Comparisons of parameters were performed using the 2-tailed Student t test or Welch test. Nppb natriuretic peptide B, BW body weight, GRK2 G protein-coupled receptor kinase 2, HW heart weight, LVEF left ventricular ejection fraction, rRNA ribosomal RNA, ns not significant. *P < 0.05.

Article Snippet: GRK2 was detected in these sections using an anti-GRK2 rabbit monoclonal antibody (1:50, ST05-60; Novus, Littleton, Colorado, United States).

Techniques: Injection, Expressing, Control, Staining

Journal: Scientific reports

Article Title: Overexpression of GRK2 in vascular smooth muscle leads to inappropriate hypertension and acute heart failure as in clinical scenario 1.

doi: 10.1038/s41598-023-34209-5

Figure Lengend Snippet: Figure 5. GRK2 overexpression inhibited vasodilation at the molecular level. The control (n = 13) and the GRK2 (n = 12) groups were exposed to saline or epinephrine respectively. (A) Schematic representation of the underlying mechanism of VSM contraction induced by epinephrine administration in the control and GRK2 groups. (B) Expression of the Mlc2 transgene determined by real-time PCR. (C) Representative images of pMLC2 immunofluorescent staining in the descending aorta. Scale bar = 100 μm. (D) Quantification of pMLC2 positive areas. Data are represented as means ± SEM. α-AR alpha-adrenergic receptor, β-AR beta- adrenergic receptor, cAMP cyclic adenosine monophosphate, GRK2 G protein-coupled receptor kinase 2, IP3 inositol triphosphate, MLC2 myosin light chain 2, MLCK myosin light chain kinase, MLCP myosin light chain phosphatase, PKA protein kinase A, pMLC2 phosphorylated myosin light chain 2, rRNA ribosomal RNA, VSM vascular smooth muscle, ns not significant. **P < 0.01.

Article Snippet: GRK2 was detected in these sections using an anti-GRK2 rabbit monoclonal antibody (1:50, ST05-60; Novus, Littleton, Colorado, United States).

Techniques: Over Expression, Control, Saline, Expressing, Real-time Polymerase Chain Reaction, Staining

Journal: Genetics and molecular research : GMR

Article Title: Increased survivin expression and its association with clinical parameters of congenital choledochal cysts.

doi: 10.4238/gmr.15028032

Figure Lengend Snippet: Figure 1. Immunohistochemical staining results for the protein expression of survivin. The cytoplasm showed high survivin expression, which presented as granular form, with yellow or dark brown staining, while the nuclei were hardly stained (A. cytoplasm, 200X; B. nuclei, 400X).

Article Snippet: The primary mouse anti-human survivin monoclonal antibody was purchased from Wuhan Boster Biological Engineering Co., Ltd. (China).

Techniques: Immunohistochemical staining, Staining, Expressing